中国现代医学杂志2005，Vol.15Issue(19)：2889-2893,5.
缺血预适血减轻大鼠后肢缺血再灌注后肺损伤的实验研究
Ischemic preconditioning attenuated lung injury following ischemia- reperfusion in hind limbs of rats
杨军 1胡新华 1刘程伟 1张志深 1张强1
作者信息
摘要
Abstract
[Objective] To study the effect of ischemic preconditioning(IPC) on the lung injury following ischemia-reperfusion (I/R) in the hind limbs of rats. [Methods] Rat model of hind limbs I/R injury was established by cross-clamping below renal artery for 4 hours. Sixty rats were divided into 4 groups: sham operation group (Group Ⅰ,n =6), ischemia group (Group Ⅱ, n =6), I/R group (Group Ⅲ, n =24), IPC+I/R group (GroupⅣ, n =24). The last two groups received 4 hours of ischemia and then 4 hours, 12 hours, 24 hours or 48 hours of reperfusion. The tissue morphology, wet-to-dry weight (W/D) ratio, malondialdehyde (MDA) and myeloperoxidase (MPO) content in the lung were observed. mRNA expression of ICAM-1 in the lung was also determined by RT-PCR or in situ hybridization.The protein product was detected by western blot. [Results] In group Ⅲ, PMN count, W/D ratio, MDA and MPO content in lung increased significantly (P <0.01). mRNA and protein expression of ICAM-1 in the lung increased significantly as compared with group ⅠorⅡ (P <0.01). In group Ⅳ, the changes were meliorated significantly as compared with group Ⅲ (P <0.01). [Conclusion] IPC can attenuate lung injury following I/R in the hind limbs of rats, which maybe accomplished by the inhibition of adhesion and infiltration of PMN after the expression of ICAM1 in the lung.关键词
缺血预适应/缺血再灌注/肺损伤Key words
ischemic preconditioning/ischemia-reperfusion/lung injury分类
医药卫生引用本文复制引用
杨军,胡新华,刘程伟,张志深,张强..缺血预适血减轻大鼠后肢缺血再灌注后肺损伤的实验研究[J].中国现代医学杂志,2005,15(19):2889-2893,5.
