中华医学杂志（英文版）2003，Vol.116Issue(2)：292-296,5.
Effects of propofol, midazolam and thiopental sodium on outcome and amino acids accumulation in focal cerebral ischemia-reperfusion in rats
Effects of propofol, midazolam and thiopental sodium on outcome and amino acids accumulation in focal cerebral ischemia-reperfusion in rats
陈莲华 1贡沁燕 2肖常思1
作者信息
- 1. Department of Anesthesiology, The Ophlhalmology and Otorhinlaryngology Hospital, Fudan University, Shanghai 200031, China
- 2. Department of Pharmacology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China
- 折叠
摘要
Abstract
Objective To investigate the effects of propofol, midazolam and thiopental sodium on outco mes and amino acid accumulation in focal cerebral ischemia-reperfusion in rats .Methods Male Sprague Dawley (SD) rats were scheduled to undergo 3-hour middle cerebral artery occlusion by intraluminal suture and 24-hour reperfusion. Neurologic outcomes were scored on a 0-5 grading scale. Infarct volume was shown with triphenyltetrazolium c hloride staining and measured by an image analysis system. Concentrations of va rious amino acids (aspartate, glutamate, glycine, taurine, and gama-aminobutyri c acid) were measured after 3 hours of reperfusion using high performance liquid chromatography. Propofol, midazolam and thiopental sodium were given intraperi toneally at the beginning of reperfusion.Results Both propofol and midazolam attenuated neurological deficits and reduced infarct and edema volumes. Propofol showed better neurological protection than midazol am while thiopental sodium did not exhibit any protective effect. Both propofol and midazolam decreased excitatory amino acids accumulation, while propofol inc reased gama-aminobutyric acid accumulation in ischemic areas in reperfusion. Conclusion Propofol and midazolam, but not thiopental sodium, may provide protective effect s against reperfusion induced injury in rats subjected to focal cerebral ischemia. This neurological protection may be due to the acceleration of excitatory am ino acids elimination in reperfusion. 关键词
cerebral ischemia/reperfusion/amino acids/propofol/midazolam/thiopental sodiumKey words
cerebral ischemia/reperfusion/amino acids/propofol/midazolam/thiopental sodium分类
医药卫生引用本文复制引用
陈莲华,贡沁燕,肖常思..Effects of propofol, midazolam and thiopental sodium on outcome and amino acids accumulation in focal cerebral ischemia-reperfusion in rats[J].中华医学杂志（英文版）,2003,116(2):292-296,5.
