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Variation and Characterization Analysis of Partial Fragment of Fibroin Gene From Silkworm, Antheraea pernyi

Li Wenli(李文利) Jin Liji An Lijia

高技术通讯(英文版)2003,Vol.9Issue(3):29-32,4.
高技术通讯(英文版)2003,Vol.9Issue(3):29-32,4.

Variation and Characterization Analysis of Partial Fragment of Fibroin Gene From Silkworm, Antheraea pernyi

Variation and Characterization Analysis of Partial Fragment of Fibroin Gene From Silkworm, Antheraea pernyi

Li Wenli(李文利) 1Jin Liji 1An Lijia1

作者信息

  • 1. Department of Bioengineering Chemistry, Dalian University of Technology, Dalian 116023, P. R. China
  • 折叠

摘要

Abstract

A 1.4Kb DNA fragment containing 3' flanking sequence of fibroin gene of silkworm, Antheraea pernyi, was obtained from the silk gland's mRNA of 5th larva. Analysis of this sequence with another A.pernyi fibroin protein (accession No. D83241) revealed that it consists of a completely open reading frame (ORF), which includes 14 polyalanine-containing units (motifs) and 100bp 3'-UTR. The sequence of the predicted amino acid reveals the highest level of overall identity (90%) with D83241. It was found that it loses a repeat region at the upstream of TAA codon and some mutations. A putative polyadenylation signal AATAAA tail was found in position 1300, which follows the termination codon.

关键词

fibroin/Antheraea pernyi/analysis/Chi-like sequence

Key words

fibroin/Antheraea pernyi/analysis/Chi-like sequence

分类

生物科学

引用本文复制引用

Li Wenli(李文利),Jin Liji,An Lijia..Variation and Characterization Analysis of Partial Fragment of Fibroin Gene From Silkworm, Antheraea pernyi[J].高技术通讯(英文版),2003,9(3):29-32,4.

基金项目

Supported by the Natural Science Foundation of Liaoning province (Grant No. 418064) (Grant No. 418064)

高技术通讯(英文版)

OAEI

1006-6748

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