作物学报2001，Vol.27Issue(1)：29-34,6.
水稻抗白叶枯病基因Xa21转基因水稻及其杂交稻研究
Study on Resistance Gene to Bacterial Blight Xa21 Transgenic Rice and Their Hybrid Combinations
摘要
Abstract
Xa21 gene and hph gene were co-transferred into Chinese 3-linehybrid rice lines Minghui 63(restorer line) and Wan B (maintainence line )by biolistics gene transfer method. A total of 5 transgenic rice lines with enhanced resistance against rice bacterial blight (BB) were obtained. Four of the transgenic lines were found to have only Xa21 gene integrated into the genome,and the other one have both Xa21 and hph integrated. Molecular analysis to the transgenic plants (up to six generations) showed that Xa21 gene had been integrated into the plant genome. The offsprings inherited the resistance from Xa21 stable. The Xa21 transgenic rice plants have been used as the restorer line for 2-line hybrid combinations. F1 of some combinations were found to show strong BB resistance and high yield-increasing potentiality.关键词
Xa21基因/转基因水稻/杂交稻分类
农业科技引用本文复制引用
吴家道,贾士荣..水稻抗白叶枯病基因Xa21转基因水稻及其杂交稻研究[J].作物学报,2001,27(1):29-34,6.基金项目
本项研究前期工作得到美国Rogor N Beachy博士的帮助， 他现在工作单位是Donald Danforth PlantScience Center (St. Louis, Mo.)， 同时也得到洛克菲勒基金(Rockefeller Foundation)的资助， 在此一并致谢。 （St. Louis, Mo.）
