首页|期刊导航|中华医学杂志（英文版）|Relationship between apolipoprotein E, D10S1225 polymorphisms and late-onset Alzheimer's disease
中华医学杂志（英文版）2006，Vol.119Issue(4)：294-299,6.
Relationship between apolipoprotein E, D10S1225 polymorphisms and late-onset Alzheimer's disease
Relationship between apolipoprotein E, D10S1225 polymorphisms and late-onset Alzheimer's disease
摘要
Abstract
Background There were some papers published in the Jonrnal of Science, December 2000 suggesting that one or more important susceptibility genes for late onset Alzheimer's disease (LOAD) were located on the long arm of chromosome 10. Linkage analysis showed maximum lod score close to D10S1225 loci, which indicated the loci might contribute to the etiology of Alzheimer's disease (AD). Methods Fifty-nine LOAD patients and 107 controls were recruited. Apolipoprotein E (ApoE) genotypes were determined by reverse dot blotting hybridization assay. The D10S1225 was genotyped by 12% nondenaturing polyacrylamide gels electrophoresis and analyzed by silver staining. Statistical analysis was used to compare genotype and allele distributions between LOAD group and control group for ApoE and D10S1225 polymorphisms.Results ApoE ε4 was significantly higher in LOAD group in comparison with the control group(χ2=6.530, P =0.011). Seven different alleles of D10S1225 have been identified. The length of these gene fragments were 178 bp, 181 bp, 184 bp, 187 bp, 190 bp, 193 bp, and 196 bp, respectively. A total of 21 different genotypes were observed. There was no relationship between D10S1225 polymorphism and LOAD (χ2=4.488, P>0.05). Conclusion This study suggests that ApoEε4 is a risk factor for LOAD, however, the results indicated that there is not any possible linkage for disequilibria with a nearby AD risk gene near D10S1225.关键词
apolipoprotein E/chromosome 10/polymorphism/Alzheimer's diseaseKey words
apolipoprotein E/chromosome 10/polymorphism/Alzheimer's disease分类
医药卫生引用本文复制引用
ZHANG Tai-song,WANG Hua-qiao,WANG Wei-yi,HE Yun-shao ,HUANG Shao-kuan..Relationship between apolipoprotein E, D10S1225 polymorphisms and late-onset Alzheimer's disease[J].中华医学杂志（英文版）,2006,119(4):294-299,6.基金项目
This study was supported by a grant from the Natural Sciences Foundation of China (No. 30470904). （No. 30470904）
