首页|期刊导航|中国海洋湖沼学报(英文版)|The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallop Chlamys farreri
中国海洋湖沼学报(英文版)2006，Vol.24Issue(3)：295-299,5.
The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallop Chlamys farreri
The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallop Chlamys farreri
摘要
Abstract
To compare genetic markers for population genetics analysis, allozyme electrophoresis and random amplified polymorphic DNA (RAPD) were used to detect the genetic structure of scallop Chlamys farreri population. Thirteen enzymes (MDH, ME, IDH, GPI, PGM, PEP-LG, PEP-PP, ACP, AK, PK, AAT, SOD, EST) in three buffer systems (TC, pH6.9; TMME, pH 7.4; and EBT, pH8.9) were selected and 22 loci were used for the analysis, among them 7 loci (Gpi, Pgm, Pep-LG-1, Pep-PP, Aat-2, Est-2, Est-3) were polymorphic which attributed 31.82% to the total. The average of heterozygosity was 0.113 and most of the studied loci showed heterozygote deficiencies. The same specimens were investigated using 10 arbitrarily selected primers (10-base). Twenty two of 54 RAPD fragments were polymorphic with average heterozygosity of 0.194. The result indicated that the two types of markers reflected a consistent trend in the parameter values of genetic diversity of the population, but RAPD revealed more information of genetic variation than allozyme electrophoresis.关键词
Allozyme/RAPD/Chlamys farreri/population/genetic structureKey words
Allozyme/RAPD/Chlamys farreri/population/genetic structure分类
生物科学引用本文复制引用
LIU Baozhong,YOU Feng ,DONG Bo ,XIANG Jianhai..The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallop Chlamys farreri[J].中国海洋湖沼学报(英文版),2006,24(3):295-299,5.基金项目
Supported by Chinese Basic Research Project (G1999012007) and National Natural Science Foundation of China (No. 39700017). （G1999012007）
