中国实用妇科与产科杂志2001，Vol.17Issue(5)：297-298,2.
应用荧光原位杂交技术对胚胎植入前行21-三体检查的研究
Detection of trisomy 21 by fluorescent in-situ hybridization for preimplantation genetic diagnosis
曲文玉 1谭季春 1姜平 1卓英梅 1蒋丽 1付民1
作者信息
摘要
Abstract
Objective To avoid transferring embryo with chromosomal aberration and improve quality of IVF-ET.Methods Our research was performed by fluorescent in-situ hybridization(FISH) for preimplantation diagnosis of trisomy 21 in 20 couples who were over 35 years old.The special DSCR Cosmid DNA probe was applied.Results It was successful to analyse chromosomes from a single cell in 10 of 20 couples.There were normal embryos in 8 of 10 couples and their embryos were transferred.2 of 8 couples had pregnancy,but one miscarriage occurred and the other was in a normal pregnancy.Trisomy 21 was detected in 2 of 10 couples and no embryo was transferred.Conclusion It is necessary to perform preimplantation genetic diagnosis for IVF patients of advanced maternal age.关键词
荧光原位杂交/体外受精-胚胎移植/植入前/21-三体分类
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曲文玉,谭季春,姜平,卓英梅,蒋丽,付民..应用荧光原位杂交技术对胚胎植入前行21-三体检查的研究[J].中国实用妇科与产科杂志,2001,17(5):297-298,2.
