医学分子生物学杂志2009，Vol.6Issue(4)：297-301,5.DOI:10.3870/j.issn.1672-8009.2009.04.004
miRNA-20a促进卵巢癌细胞OVCAR3的转移
microRNA-20a Promotes Metastasis of OVCAR3 Ovarian Cancer Cells
摘要
Abstract
Objective To detect the effect of miR-20a on metastasis.Methods The effects of antisense oligonucleotide and small interfering RNA of miR-20a were confirmed by Real-time PCR. miR-20a was either blocked or overexpressed in human ovarian cancer cell line OVCAR3. Cell proliferation was detected with MTT assay and soft-agarose formation assay. And the impact of miR-20a on cell invasion was detected with transwell invasion assay.Results When miR-20a was suppressed, both colony formation activity and invasion activity of OVCAR3 were inhibited. On the contrary, when miR-20a was overexpressed, OVCAR cells were promoted to form colony and become invasive. Cell growth of OVCAR3 was not subject to either suppression or overexpression of miR-20a.Conclusion miR-20a contributes to metastasis of OVCAR3 cells.关键词
miR-20a/转移/OVCAR3细胞/侵袭实验Key words
miR-20a/metastasis/OVCAR3/transwell分类
医药卫生引用本文复制引用
韩喆,刘艳坤,姜洁纯,刘民,李欣,汤华..miRNA-20a促进卵巢癌细胞OVCAR3的转移[J].医学分子生物学杂志,2009,6(4):297-301,5.基金项目
国家自然科学基金(No.30873017),天津市自然科学基金重点基金项目(No.08JCZDJC23300)This work supported by grants from the National Natural Science Foundation of China(No.30873017)，the Key Program of the Natural Science Foundation of Tianjing(No.08JCZDJC23300) （No.30873017）
