分析化学2001，Vol.29Issue(3)：299-302,4.
吸附固定相电色谱和动态改性电色谱的手性分离
Chiral Separation by Capillary Electrochromatography with PhysicallyAdsorbed Stationary Phase and Dynamically Modified Stationary Phase
叶明亮 1邹汉法 1雷政登 1吴仁安 1倪坚毅1
作者信息
- 1. 中国科学院大连化学物理研究所,国家色谱研究与分析中心,
- 折叠
摘要
Abstract
A novel mode of chiral separation in electrochromatography (CEC) with dynamically modified stationary phase (DMS-CEC) was presented. The capillary column was packed with strong anionic exchange stationary phase, the sulfated β-cyclodextrin (S-CD), which was added in the mobile phase, dynamically adsorbed to the packing surface and a new layer of chiral stationary phase was formed. The separation of enantiomer was based on their different interaction with the new stationary phase. The enantionmers of tryptophan, atropine and verapamil were successfully separated in this system with resolution of 2.06, 10.1 and 1.96, and the column effeciency for the enantiomers were varied from 85000 plates/m to 412000 plates/m. The relative standard deviation (RSD) of void time and the tryptophan enantiomers′ migration time for 17 consecutive runs were 0.5%, 0.6% and 0.7%, respectively. Enantiomer separation by capillary electrochromatography with adsorbed bovine serum albumin (BSA) and sulfated cyclodextrin (S-CD) as chiral stationary phases were also studied. The resolution for tryptophan enantiomers in the two systems were 3.86 and 2.97, respectively. It was found that the superiority of DMS-CEC over the adsorbed S-CD column CEC was that better repeatability could be obtained in DMS-CEC.关键词
毛细管电色谱/动态改性/吸附固定相/手性分离分类
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叶明亮,邹汉法,雷政登,吴仁安,倪坚毅..吸附固定相电色谱和动态改性电色谱的手性分离[J].分析化学,2001,29(3):299-302,4.
