激光生物学报2007，Vol.16Issue(3)：299-303,5.
粘质沙雷氏菌几丁质酶chiB基因的克隆与序列分析
Cloning and Sequence Analysis of Serratia marcescens Chitinase chiB Gene
摘要
Abstract
The genome DNA was extracted from serratia marcescens by improved method .A special fragment about 1 500 bp length was cloned from serratia marcescens genome DNA by Polymerasw Chain Reaction (PCR) amplification. Vector Puc-CHIb was constructed through ligating the fragment into the plasmid pUC18 and transformed into E. Coli DH5α. Through screening of recombinants and sequence analysis of it, the result showed that the cloned DNA fragment was chitinase chiB gene of Serratia marcescens which was the same as reported.关键词
几丁质酶/chiB基因/克隆/序列分析Key words
chitinase/chiB gene/cloning/sequence analysis分类
生物科学引用本文复制引用
叶辉,程备久,朱苏文,甘德芳,冯春..粘质沙雷氏菌几丁质酶chiB基因的克隆与序列分析[J].激光生物学报,2007,16(3):299-303,5.基金项目
Scientifical Item of Education Department of Anhui Province(No.2006KJ213B) （No.2006KJ213B）
