湖南农业大学学报（自然科学版）2009，Vol.35Issue(3)：299-303,313,6.
草鱼细胞凋亡抑制蛋白基因cDNA的克隆与表达分析
Molecular cloning, characterization, and expression analysis of full-length cDNA of inhibitor of apoptosis protein gene from grass carp
摘要
Abstract
A full-length cDNA of inhibitor of apoptosis protein was cloned using rapid amplification cDNA ends (RACE). The sequence analysis shows that the full-length of inhibitor of apoptosis protein cDNA from grass carp was 2 093 bp,which contained a 1 944 bp of open reading frame (ORF) encoding a inhibitor of apoptosis protein precursor of 647 amino acid,flanked by a 25 bp of 5′-untranslated region (UTR) and a 124 bp of 3′-UTR;the analysis show that the amino acid sequence may not contain a signal peptide, and has a high similarity to that of zebrafish, followed by lctalurus punctatus; In the evolutionary tree, the grass carp was clustered with zebrafish and lctalurus punctatus. The expression of inhibitor of apoptosis protein gene in different tissues from bacteria-induced or healthy grass carp were detected by semi-quantitative RT-PCR. The results indicated that: IAP is expressed in abundance in the brain, intestines and kidney in normal tissues,and there was a little expression in liver,heart,but gill,and muscle with minimum expression. However, all differences were not significant. After infected with bacterial Aeromonas hydrohila 24 h,the mRNA level of all the tissues dropped averagely by about 26 percent especially in liver and muscles;After infection 48 h,the mRNA level in all tissues increased by about 4 percent,that in the heart,liver and gills increased by 8 percent,16 percent and 19 percent respectively;The results indicated that inhibitor of apoptosis protein may be involved in the immune response for Aeromonas hydrophila infection.关键词
草鱼/凋亡抑制蛋白/快速扩增cDNA末端/表达分析Key words
grass carp/inhibitor of apoptosis protein/rapid amplification cDNA end/expression分类
农业科技引用本文复制引用
左振华,唐建州,张钊,唐春华,陈韬,张东裔..草鱼细胞凋亡抑制蛋白基因cDNA的克隆与表达分析[J].湖南农业大学学报（自然科学版）,2009,35(3):299-303,313,6.基金项目
国家教育部新世纪优秀人才支持项目(NCET- 05-0715) （NCET- 05-0715）
湖南省教育厅优秀博士论文奖励基金 （）
湖南农业大学稳定人才基金(03WD02) （03WD02）
