激光生物学报2002，Vol.11Issue(4)：301-304,4.
小麦总RNA的快速提取
Rapidly Isolating Total RNA from Wheat
摘要
Abstract
RNeasy Kits are used to isolate total RNA from small quantities of wheat. They provide a fast and simple method to make multiple, simultaneous processing of a wide variety of biological samples possible in less than 30 minutes. The use of toxic substance such as phenol and/or chloroform is replaced by the RNeasy procedure. The purified RNA is ready for use in standard and downstream applications such as RT-PCR, polyA+RNA selection, differential display, Northern dot and slot blotting, primer extension, cDNA synthesis, expression array and expression-chip analysis. We have successfully isolated total RNA from wheat with RNeasy Kit for many times, three of which were analyzed in this paper. The value of OD260nm/OD280nm fell in the range from 1.7 to 1.9 and that of OD260nm/OD230nm is larger than 2.0, which indicated that the purity of RNA was satisfactorily high ( RNA was not contaminated by proteins or benzene phenol etc.)关键词
总RNA提取/小麦/抗盐基因Key words
total RNA isolation/wheat/salt-resistant gene分类
生物科学引用本文复制引用
龚莉桂,沈龙珠,徐子勤..小麦总RNA的快速提取[J].激光生物学报,2002,11(4):301-304,4.基金项目
陕西省自然科学基金重点项目 （）
陕西省高校省级重点实验室重点科研项目. （）
