国际眼科杂志2007，Vol.7Issue(2)：301-304,4.
大鼠视网膜缺血再灌注后HIF-1α的表达与视网膜细胞凋亡的研究
HIF-1α expression and retinal cell apoptosis in rat retina ischemia-reperfusion injury
张薇 1刘宁宁 1许建华 1刘哲丽1
作者信息
- 1. 110001,中国辽宁省沈阳市,中国医科大学附属第一医院眼科
- 折叠
摘要
Abstract
AIM:Investigating the expression of HIF-1α,apoptosis of retinal cells and the role of HIF-1α in apoptosis in rats' retinal ischemia-reperfusion injury.METHODS:The rat model of experimental retinal ischemia-reperfusion injury was established by increasing the intraocular pressure to 110mmHg(1kPa=7.5mmHg)in rat eyes.At different time points of post ischemia,the expression of HIF-1α of the retina was detected by immunohistochemicalstaining,and apoptosis of the retinal cell was detected by terminal deoxynudeotidyl transferase medialed deoxyuridine triphosphatebiotin nick end labeling(TUNEL).RESULTS:HIF-1α appeared in the cells of retinal ganglion layer and inner nuclear layer at 2 hours after ischemia.The expression reached to a peak,12 hours after retinal ischemia-reperfusion,then the expression was dedined.The apoptotiC Cells were mainly in inner nuclear layer and could be detected at the 12th, 24th and 48th hour after ischemia,the peak value was the group of 24th hour.CONCLUSION:Expression of HIF-1α in the rats' retina is greatly enhanced after ischemia-reperfusion,which may be involved in the retinal injury:the injury of retinal neurons Occurs partly in the form of apoptosis.The expression of HIF-1α may play an important role in cell apoptosis.关键词
缺血再灌注/视网膜/HIF-1α/凋亡Key words
ischemia reperfusion/retina/HIF-1α/apoptosis分类
医药卫生引用本文复制引用
张薇,刘宁宁,许建华,刘哲丽..大鼠视网膜缺血再灌注后HIF-1α的表达与视网膜细胞凋亡的研究[J].国际眼科杂志,2007,7(2):301-304,4.
