中国药科大学学报2001，Vol.32Issue(1)：30-33,4.
人血浆中瑞格列奈HPLC法测定及其药代动力学研究
Determination of Repaglinide in Plasma by HPLC and Its Pharmacokinetics in Men
季慧芳 1谢林 1严铭娟 1张琳 1黄鑫 1刘晓东1
作者信息
- 1. 中国药科大学药代研究中心， 南京 210009
- 折叠
摘要
Abstract
AIM: To establish a reversed phase HPLC method for determinationof repaglinide and to study pharmacokentics of repaglinide in men. METHODS: After acidified, the drug was extracted from plasma with acetate ether. Shim-pack CLC-ODS C18 column was used. Mobile phase consisted of 27% 0.10 mol/L citrate acid-sodium acetate buffer(pH=4.0) and 73% methanol. The eluted peaks were detected by UV detector at 243 nm. RESULTS: The recoveries of repaglinide from plasma were larger than 90% and RSD% of intra-day and inter-day were smaller than 15%. Calibration curves were linear over 2.5～100.0 ng/ml(r=0.9992) and the minimum detection concentration was 2.5 ng/ml. The pharmcokinetics of repaglinide in 22 healthy subjects was studied. After oral administration of 4 mg repaglinide tablet, the pharmacokinetic parameters were estimated as follows: T1/2, 0.83±0.31 h, Tmax, 0.75±0.41h, Cmax, 53.32±24.94 ng/ml, MRT 1.58±0.41 h, and, 74.95±30.57 ng·h/ml. CONCLUSION: A reliable and rugged simultaneous HPLC assay for repaglinide was developed. The assay method was convenient for clinical laboratory.关键词
瑞格列奈/HPLC/药代动力学分类
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季慧芳,谢林,严铭娟,张琳,黄鑫,刘晓东..人血浆中瑞格列奈HPLC法测定及其药代动力学研究[J].中国药科大学学报,2001,32(1):30-33,4.
