中华医学杂志（英文版）2001，Vol.114Issue(1)：30-34,5.
全仅式维甲酸(ATRA)和三氧化二砷(As2O3)对APL细胞组织因子表达的影响
Effect of all-trans retinoic acid and arsenic trioxide on tissue factor expression in acute promyelocytic leukemia cells
郭为民 1王鸿利 1赵维莅 1诸江 1璩斌 1王学锋1
作者信息
- 1. 上海第二医科大学瑞金医院上海血液学研究所上海 200025
- 折叠
摘要
Abstract
Objective To study the effect of all-trans retinoic acid (ATRA) and arsenic trioxide (As2O3) on tissue factor (TF) expression and procoagulant activity (PCA) of acute promyelocytic leukemia (APL) cells in vivo and in vitro.
Methods PCA from freshly isolated APL blasts from APL patients treated with ATRA or As2O3 was detected using a one-stage clotting assay. TF antigen was detected by ELISA and TF mRNA by RT-PCR. The maturation sensitive (NB4) or resistant subclones (NB4-R1) of the promyelocytic NB4 cell line, as well as U937 cells infected with pMSCV-PML-RARa treated with or without ATRA or As2O3, were also examined.
Results Both ATRA and As2O3 can down-regulate the TF antigen, its mRNA transcription and membrane PCA of APL cells in vivo and in vitro, in a time-dependent manner. The TF antigen level in PML-RARa+ U937 cells was significantly higher than that in U937 cells infected with retrovirus vector. Both ATRA and As2O3 can also down-regulate the TF antigen in U937 cells transfected with or without PML-RARa.
Conclusion Tissue factor expression and PCA in APL cells may be down-regulated by ATRA and As2O3. By down-regulating TF expression, As2O3 might also be used to improve the DIC-related hemorrhage in APL. Our data indicate that elevated TF antigen in PML-RARa+ U937 may be related to the fusion protein PML-RARa. The down-regulating effect of ATRA and As2O3 on TF expression in U937 cells might not involve this fusion protein.关键词
急性早幼粒细胞性白血病全反式维甲酸三氧化二砷组织因子促凝活性弥散性血管内凝血Key words
acute promyelocytic leukemia/all-trans retinoic acid/arsenic trioxide/tissue factor/procoagulant activity/disseminated intravascular coagulation分类
医药卫生引用本文复制引用
郭为民,王鸿利,赵维莅,诸江,璩斌,王学锋..全仅式维甲酸(ATRA)和三氧化二砷(As2O3)对APL细胞组织因子表达的影响[J].中华医学杂志（英文版）,2001,114(1):30-34,5.
