农药学学报2001，Vol.3Issue(1)：30-35,6.
棉铃虫谷胱甘肽S-转移酶的活性分布和发育期变化及植物次生物质的诱导作用
Glutathione S-Transferases(GSTs) in Helicoverpa Armigera:Subcellular and Tissue Distribution of Activity,Developmental Changes and Induction of Allelochemicals
摘要
Abstract
The Activity of GSTs in different tissues, developmental stages, subcellular fractions and induction by allelochemicals was determined in cotton bollworm, Helicoverpa armigera (Hübner). GSTs activity toward 1-chloro-2,4-dinitrobenzene (CDNB) was detected in the egg. It increased throughout the larval stages and up to the highest in the sixth instar larvae. The activity of GSTs from the larvae fed on artificial diet containing 50 mg/kg rutin or 2-tridecanone reached the peak at the fifth instar. Enzyme activity was found in fat body, midgut, head and cuticle of the sixth instar larvae. The GSTs activity in fat body was the highest in above tissues. The Km values for CDNB conjugation varied with different tissues, and the Km values of GSTs in the midgut was the lowest (8.5 mmol/L). The supernatant fraction possessed more than 90% of total activities of GSTs in the homogenate of midguts of the fifth instar larvae. The GSTs activity in microsome fraction from the third instar larvae induced by rutin was obviously lower than that fed only on artificial diet, but the enzyme activity in supernatant fraction increased.关键词
棉铃虫/谷胱甘肽S-转移酶/植物次生物质/诱导作用分类
农业科技引用本文复制引用
张常忠,高希武,郑炳宗..棉铃虫谷胱甘肽S-转移酶的活性分布和发育期变化及植物次生物质的诱导作用[J].农药学学报,2001,3(1):30-35,6.
