陕西科技大学学报（自然科学版）2007，Vol.25Issue(2)：30-35,6.
响应面法优化磁性固定化纤维素酶的制备
PREPARING MAGNETIC IMMOBILIZED CELLULASE BASED ON RESPONSE SURFACE METHODOLOGY
摘要
Abstract
The conditions such as immobilized time, pH value, buffer solution concentration and the amount of cellulase for preparing magnetic immobilized cellulase were optimized by means of the response surface methodology (RSM), the activity of magnetic immobilized cellulase was chosen as the response value. And the mathematical regression model between different variables and the activity of magnetic immobilized cellulase was obtained using regression equation. Determination coefficient (R2) was 0.902 4, lack of fit did not exist. The results demonstrated the optimum parameters of preparing magnetic immobilized cellulase were: immobilized time of 11.98 h, pH value of 5.2, buffer solution concentration of 0.066 mol·L-1 , the amount of cellulase used of 202.7 mL. Under the optimum parameters, the maximal activity of magnetic immobilized cellulase was 28. 457 44 IU·g-1.关键词
响应面分析/磁性固定化纤维素酶/酶活力/固定化Key words
magnetic immobilized cellulase/enzyme activity/response surface methodology分类
生物科学引用本文复制引用
寇灵梅,李冰,郭祀远,李琳,高斯哲..响应面法优化磁性固定化纤维素酶的制备[J].陕西科技大学学报（自然科学版）,2007,25(2):30-35,6.基金项目
国家自然科学基金青年基金(20306007)、广东省自然科学基金重点项目(04105934)和国家自然科学基金重点项目(20436020) （20306007）
