中国农业科学（英文版）2002，Vol.1Issue(1)：30-35,6.
Study on the Factors Influencing the Efficiency of Wheat Transformation
Study on the Factors Influencing the Efficiency of Wheat Transformation
摘要
Abstract
Wheat transformation efficiency is closely related to several factors such as receptor genotype, constructed plasmid and selection procedure after bombardment or co-cultivation. In our study, several kinds of antibiotics, which were normally used in plant transformation to the selection genes of nptⅡ, bar and hpt,were tested for the optimal concentrations for wheat transformation. The results showed that 25 - 50mg/L of geneticin (G418) was suitable for the selection of nptⅡ, kanamycin or neomycin was not suitable for use. 3 5mg/L of phosphinothricin (PPT) or biolaphos could be used for the selection of bar, 100 - 150mg/L of hygromycin for the selection of hpt. Yangmai 158 and Yangmai 10 with high tissue culture response and good agronomic characteristics were screened from 25 potential Chinese wheat cultivars. The concentration changing of selectable agent in selection medium was helpful to obtain enough regeneration plantlets with strong root system.关键词
Wheat/Transformation/Selectable marker/Receptor genotype/Selection procedureKey words
Wheat/Transformation/Selectable marker/Receptor genotype/Selection procedure引用本文复制引用
YE Xing-guo,XU Hui-jun,DU Li-pu,XIN Zhi-yong..Study on the Factors Influencing the Efficiency of Wheat Transformation[J].中国农业科学（英文版）,2002,1(1):30-35,6.基金项目
This work was supported by National Transgenic Plants Fund ( J99-A-40 ) and Europe Union Cooperation Fund(ERBIC18CT96-0049). （ J99-A-40 ）
