中国组织工程研究与临床康复2009，Vol.13Issue(16)：3041-3044,4.DOI:10.3969/j.issn.1673-8225.2009.16.008
去细胞组织工程异种瓣膜支架制备方法的比较
Various methods of preparing acellular tissue-engineered xenogeneic valve stents
王鹏 1李超 1张润起 1谈磊 1孔凡华1
作者信息
- 1. 泰安市中心医院胸外科,山东省泰安市,271000
- 折叠
摘要
Abstract
BACKGROUND: Excellent Iow-antigenicity xenogeneic biological valve scaffold is the premise of constructing tissue-engineered valve by using which kind of acellular methods.OBJECTIVE: To explore the optimal preparation method of making tissue engineered heart valves by meesuring efficiency of different acellular methods and ability to preserve the matrix.DESIGN, TIME AND SETTING: The prospective randomly controlled study was performed at the Central Laboratory of Taian Central Hospital from January 2007 to June 2008.MATERIALS: Sixteen specimens of porcine aortic valves were randomly divided into control, NaCI-sodium-dodecyl-sulfate (SDS),trypsin and triton-X100 groups.METHODS: Specimens in the control group were left intact. Three test groups were decelluladzed with NaCI, trypsin andTriton-X100 respectively.MAIN OUTCOME MEASURES: The gross structure, optical and electron microscope ultrastructure of the decelluarated porcineheart valve matrix was compared. The expression of vascular endothelial cell major histocompatibility complex (MHC)- Ⅰ antigenwas detected by immunohistochemical method.RESULTS: Treatment with NaCL-SDS achieved only incomplete decellularization. The main components of extracellular matdxwere reserved completely, but the fibrous components became unclear and swelling. Treatment with trypsin removed cellscompletely, but caused serious structural alterations, with the presence of swollen collagen fiber, crude edge, widen and irregularfiber interspace. Treatment with Triton-X100 achieved both complete decelluarization and preservation of the matrix structure.Valves following treatment of NaCI-SDS, trypsin and Triton-X100 had certain immunogenicity. However, the immunogenicity ofvalves following treatment of trypsin and Triton-X100 was significantly lower compared with the treatment of NaCL-SDS.CONCLUSION: The decellularization method by Triton-X100 is effective and complete. The Triton-X100 method does not changematrix structure and has low immunogenicity.关键词
组织工程/心脏瓣膜/脱细胞方法/主要组织相容件复合体分类
医药卫生引用本文复制引用
王鹏,李超,张润起,谈磊,孔凡华..去细胞组织工程异种瓣膜支架制备方法的比较[J].中国组织工程研究与临床康复,2009,13(16):3041-3044,4.
