第一军医大学学报2001，Vol.21Issue(4)：304-306,3.
一种简便又经济的肝脏贮脂细胞分离方法
A simple and economic method for isolating hepatic lipocytes
张艺军 1杨希山 1吴平生 2刘树人3
作者信息
- 1. 第一军医大学南方医院全军消化内科研究所
- 2. 第一军医大学南方医院心血管内科,
- 3. 解放军458医院肝病研究所,
- 折叠
摘要
Abstract
Objective To establish a simple and economic method for isolating rat hepatic lipocytes. Method Perfusion of the rat liver with pronase E and collagenase in succession was performed in cycles, and the hepatic lipocytes were isolated and purified by digesting the rat liver with DNase in the presence of the above 3 enzymes, followed by 11% Necodenz density gradient centrifugation. The resulted lipocytes were subjected to routine identification procedures and viability assessment. Results The yield of the lipocytes from each rat was 2×107 to 3×107, with viability over 95% and purity above 90%. Conclusion The method adopted in this study is simple, economic and highly productive.关键词
贮脂细胞/细胞分离/细胞培养分类
医药卫生引用本文复制引用
张艺军,杨希山,吴平生,刘树人..一种简便又经济的肝脏贮脂细胞分离方法[J].第一军医大学学报,2001,21(4):304-306,3.
