南京医科大学学报（英文版）2004，Vol.18Issue(6)：304-307,4.
紫草素对人胎肾小球系膜细胞增殖、凋亡及细胞外基质的影响
Effects of Shikonin on Proliferation, Apoptosis, and Extracellular Matrix of Human Mesangial Cells
摘要
Abstract
Objective: To investigate the effect of shikonin on the proliferation, apoptosis and extracellular matrix (ECM) of human mesangial cells (MC). Methods : MC was cultured in vitro with different concentrations of glucose (30, 50, 80 mmol/L). The cell growth was observed by using MTT method and apoptosis by using an aunexin-V-Fluos.Immunohistochemical studies for Laminin (LN), Fibronectin (FN) and type Ⅳ Collagens (Col Ⅳ ) were measured. Results: Shikonin inhibited their growth ( P ＜ 0.05) and apoptosis in the glycated cultured cells. Shikonin 0.05 mmol/L significantly reduced the secretion of LN, FN and Col Ⅳ from MC (P ＜ 0.05) cultured in 30, 50 and 80 mmol/L glucose.Conclusion: Shikonin could prevent or treat diabetic nephropathy (DN) and glomerulosclerosis (GS).关键词
紫草素/肾系膜细胞/增殖/系膜基质/凋亡/肾小球硬化Key words
shikonin/mesangial cell/proliferation/extracellular matrix/apoptosis/glomerulosclerosis分类
医药卫生引用本文复制引用
李海涛,李晓冬,朱荃..紫草素对人胎肾小球系膜细胞增殖、凋亡及细胞外基质的影响[J].南京医科大学学报（英文版）,2004,18(6):304-307,4.基金项目
Supported by the Grant of Jiangsu province postPh. D. Scientific Research Grant （）
