中德临床肿瘤学杂志（英文版）2008，Vol.7Issue(5)：304-308,5.
曲古菌素A对HeLa细胞增殖及其基因表达的影响研究
Effects of trichostatin A (TSA) on growth and gene expression in HeLa cells
摘要
Abstract
Objective:To investigate the expressions of p53,RB1,Fas,c-fos,Ras,EGFR mRNA in human cervical cancer (HeLa) cell in response to the trichostatin A (TSA).Methods:We took count of HeLa cells in different incubation times with TSA (0.2 IJm/L).The result indicated that HeLa cells changed evidently when HeLa cells were incubated for 36 h.Then,we investigated the genes expression (mRNA levels) of HeLa cells after treatment for 36 h using SYBR green real-time PCR.Results:We demonstrated that trichostatin A (TSA) could make human cervical cancer (HeLa) cell morphological change and induce HeLa cell apoptosis.Furthermore,the data suggest that TSA-induced down-regulation of p53,RB1,Fas,but upregulated c-fos gene expression after treatment for 36 h,and Ras,EGFR did not show obvious response to TSA treatments.Conclusion:TSA has different effects on gene expression.关键词
TSA/ HeLa cell/ real-time PCR/ gene expressionKey words
TSA/ HeLa cell/ real-time PCR/ gene expression分类
医药卫生引用本文复制引用
Fengqiu Zhang,Huasheng Fang,Yuxiao Li,Guangyong Qin..曲古菌素A对HeLa细胞增殖及其基因表达的影响研究[J].中德临床肿瘤学杂志（英文版）,2008,7(5):304-308,5.基金项目
Supported by a grant from the National Science and Technique Foundation during the Tenth Five-Year Plan Period of China (No.2001BA302B-03).Note:Fengqiu Zhang and Huasheng Fang contribute equally to this work. （No.2001BA302B-03）
