湖南农业大学学报（自然科学版）2009，Vol.35Issue(3)：304-308,5.
黄鳝抗菌肽hepcidin基因cDNA的克隆及表达分析
Molecular cloning and expression analysis of a hepcidin gene from rice field eel (Monopterus albus)
摘要
Abstract
Based on the homology of known hepcidin,a pair of degenerate primers were designed,and a molecular cloning of a hepcidin gene from the liver of a rice field eel was conducted. The full-length cDNA of the gene (GenBank accession number:FJ436808) was identified by RT-PCR and RACE. It is 712 bp long flanked by a 133 bp of 5′-untranslated region and a 306 bp of 3′-untranslated region.and containing an ORF of 273 bases encoding a prepropeptide of 90 amino acid residues. Homologous analysis indicated that deduced amino acid sequence from rice field eel was highly homologous to hepcidin of other fishes reported,featuring 8 cysteine,being a membership of hepcidin family.Semi-quantitative RT-PCR was performed and the results demonstrated that hepcidin transcripts are highly abundant in liver,abundant in kidney,less abundant in heart,skin,brain,blood cells,intestine,spleen and stomach,and undetectable in muscle. When challenged with LPS injection 24 h,up-inducible hepcidin mRNA levels were detected in all tested organisms. The cloning of rice filed eel hepcidin provide new candidate gene for fish breeding and basis data for searching alternative therapeutants.关键词
黄鳝/抗菌肽/hepcidin基因/克隆/表达分析Key words
Monopterus albus/antimicrobial peptides/hepcidin gene/cloning/gene expression分类
农业科技引用本文复制引用
李伟,孙文秀,熊涛..黄鳝抗菌肽hepcidin基因cDNA的克隆及表达分析[J].湖南农业大学学报（自然科学版）,2009,35(3):304-308,5.基金项目
湖北省教育厅青年基金项目(Q200712005) （Q200712005）
长江大学博士启动基金(2006A0443) （2006A0443）
