中国水产科学2002，Vol.9Issue(4)：304-308,5.
太平洋牡蛎核糖体DNA转录间隔子和线粒体基因片段序列测定
Sequencing of ribosomal internal transcribed spacer regions and mitochondrial gene fragments in Crassostrea gigas
摘要
Abstract
The ribosomal internal transcribed spacer regions (ITS-1 and ITS-2) and mitochondrial gene fragments ( 16S rDNA and COI) of Crassostrea gigas were amplified via PCR, and the PCR products were ligated into T-vectors, cloned and sequenced.The nucleotide sequences of 543,791,530 and 700 bp from ITS-1,ITS-2,partial 16S rRNA gene and partial COI gene were obtained, respectively. The contents of A, T, G and C were 23.57%, 20.07%, 29.47% and 26.89% in ITS-1, 27.43%, 19.22%, 27.05% and 26.30% in ITS-2, 29.25%, 29.25% 23.02% and 18.49% in 16S rDNA, and 22.71%, 39.43%, 20.43% and 17.43% in COI in turn. The primers of ITS-1 and ITS-2 proved to be very universal in a variety of mollusk species. The potential uses of these four sequences for species identification and relevant research of several closely related oyster species were discussed.关键词
太平洋牡蛎/ITS-1/ITS-2/COI/16S rDNA/基因序列Key words
Crassostrea gigas/ITS-1/ITS-2/COI/16S rDNA/sequencing分类
生物科学引用本文复制引用
孔晓瑜,张留所,喻子牛,刘亚军,王清印..太平洋牡蛎核糖体DNA转录间隔子和线粒体基因片段序列测定[J].中国水产科学,2002,9(4):304-308,5.基金项目
Financially supported by Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture and National Science Foundation of China (396001133 and 39620260). （396001133 and 39620260）
