棉花学报2001，Vol.13Issue(5)：304-308,5.
硬脂酸脱氢酶基因ghSAD-1倒位重复构建的棉花遗传转化
Cotton Transformation Based on Inverted Repeat Construct of stearoyl-ACP △9 desaturase GeneghSAD-1
摘要
Abstract
A partial inverted repeat based gene construct was produced and cloned into the pBI-Lectin binary vector, using the strong seed specific soybean Lectin promoter and cotton stearyol-ACP △9 desaturase gene ghSAD-1.The cotton cultivar G. hirsutum cv. Coker315was then transformed by the construct through Agrobacteriurn mediated infection. A relatively high transformation frequency was obtained.Further analysis by PCR, Southern blotting and fatty acid composition analysis demonstrated that the expression or silencing effectiveness of the transgene was not in the same level among various transgenic lines, where the stearic acid content was increased from 2 percent of the control to a range of 4 ～ 38. 2 percent. These materials are valuable for the further improvement of current cottonseed oil in the future.关键词
棉子/油分/硬脂酸/基因/沉默/倒位重复Key words
cotton/seed oil/stearic acid/gene silence/inverted repeats分类
农业科技引用本文复制引用
刘任重,Qing Liu ,王留明,王家宝,杨静,Allan Green..硬脂酸脱氢酶基因ghSAD-1倒位重复构建的棉花遗传转化[J].棉花学报,2001,13(5):304-308,5.基金项目
This program is supported by Cotton Research & Development Corporation Australia and Chinese Scholarship Council. All the right reserved in Australia. （）
