南京医科大学学报（英文版）2003，Vol.17Issue(6)：304-308,5.
氧化砷对MR2细胞耐药分子表达的影响
The Effect of As2O3 on the Epression of Drug-resistance Molecule in Malignant Neoplasmas
钱晓萍 1刘宝瑞 1王立峰 1杜娟 1臧秦川1
作者信息
- 1. 南京大学医学院附属鼓楼医院肿瘤科,南京,210008
- 折叠
摘要
Abstract
Objective: To detect the action of arsenic trioxide (As2 O3 ) on the expressionof Tumor drug-resistant molecule. Methods: APL cell line MR2 resistant to all-trans retinoic acid (ATRA) was put into research, while APL cell line NB4 was used for control. The immunocytochemical assays were used to detect the expressions of P-glycoprotein ( Pgp ) and Glutathione S-transferase (GST). Results: Not only the expression of Pgp in MR2 cell linw (30%-40%) was significantly higher than that in NB4 cell linc (10%-20%) (p ＜ 0.001), but also the expression of GST in MR2 cell linc (60.4 ± 4.0)- (66.5 ± 4.4) was significantly higher than that in NB4 cell linc (28.3 ± 5.6)- (31.2 ± 5.1) ( P ＜ 0.05). As2 O3 at the concentration of 0.5-2.0μmol/l could significantly aecrease the expression of Pgp and GSTπ, but could do nothing about the expression of GSTα and GSTμ . Conclusion: The lower expression of Pgp and GSTπ might be the sensitive indications of frustrating drug-resistance, while GSTα and GSTμ might not be the case. ATRA might be the substrates of Pgp transmission and GSTπ catalysis.关键词
氧化砷/肿瘤/P-糖蛋白/谷胱甘肽S转移酶Key words
arsenic trioxide/tumor/P-glycoprotein/glutathione S-transferase分类
医药卫生引用本文复制引用
钱晓萍,刘宝瑞,王立峰,杜娟,臧秦川..氧化砷对MR2细胞耐药分子表达的影响[J].南京医科大学学报（英文版）,2003,17(6):304-308,5.
