首页|期刊导航|中国海洋湖沼学报（英文版）|Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins
中国海洋湖沼学报（英文版）2007，Vol.25Issue(3)：304-309,6.
Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins
Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins
摘要
Abstract
An actin gene (CfACT1) was cloned by using RT-PCR, 3' and 5'RACE from hemocytes of the sea scallop Chlamys farreri. The full length of the transcript is 1535 bp, which contains a long 3' un-translated region of 436bp and 59bp of a 5' un-translated sequence. The open reading frame encodes a polypeptide of 376 amino acids. Sequence comparisons indicated that CfACT1 is more closely related to vertebrate cytoplasmic actins than muscle types. Phylogenetic analysis showed that molluscan actins could be generally divided into two categories: muscle and cytoplasmic, although both are similar to vertebrate cytoplasmic actins. It was also inferred that different isotypes existed in muscle or cytoplasma in mollusks. The genomic sequence of CfACT1 was cloned and sequenced. Only one intron was detected:it was located between codons 42 and 43 and different from vertebrate actin genes.关键词
Chlamys farreri/actin/clone/phylogenetic analysis/intronKey words
Chlamys farreri/actin/clone/phylogenetic analysis/intron分类
生物科学引用本文复制引用
..Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins[J].中国海洋湖沼学报（英文版）,2007,25(3):304-309,6.基金项目
Supported by the National Basic Research Program of China (973 Program,No. G1999012012) and the Key Laboratory of Mariculture, Ministry of Education (No. 2004024) （973 Program,No. G1999012012）
