中国当代儿科杂志2003，Vol.5Issue(4)：306-310,5.
缬沙坦对阿霉素肾病肾硬化大鼠细胞凋亡及Fas和FasL表达的影响
Effects of Valsartan on Apoptosis and Expression of Fas and FasL in the Kidneys of Rats with Nephrotic Glomerulosclerosis Induced by Adriamycin
韩子明 1邢燕 1王宏伟 1梁秀玲 1周建华1
作者信息
- 1. 华中科技大学同济医院儿科,湖北,武汉,430030
- 折叠
摘要
Abstract
Objective To inquire into the effects of angiotensin Ⅱ type 1 receptor antagonist (AT1RA) valsartan on apoptosis and the expression of apoptosis correlated protein Fas and FasL in the kidneys of rats with nephrotic glomerulosclerosis induced by adriamycin. Methods Thirty six SD rats were randomly divided into model group, treatment group and control group. The model was established by uninephrectomy and injection of adriamycin. In the treatment group, valsartan (20 mg/kg) was delivered daily by gavage for 12 weeks. The same amount of normal saline was delivered by gavage in the control and model groups. Apoptosis was examined by means of terminal-deoxynucleotidyl transferase mediated d-UTP nick end labeling (TUNEL). Glomerular apoptotic index (GAI) and renal tubule apoptotic index (TAI) were calculated. Immunohistochemistry was utilized to detect the expression of Fas and FasL. The morphological changes were observed under optic microscope and the glomerulosclerosis index (GSI) was determined. Results Compared with the control group, the GSI, GAI, TAI and the expression of Fas and FasL in model and treatment groups were stronger (P<0.01). Compared with model group, they decreased in treatment group (P<0.01). Conclusions Valsartan may suppress the excessive apoptosis of kidney cells by lowering the expression of Fas and FasL so as to postpone the process of glomerulosclerosis.关键词
血管紧张素Ⅱ1型受体拮抗剂/缬沙坦/阿霉素肾病/细胞凋亡/Fas/FasLKey words
Angiotensin Ⅱ type 1 receptor antagonist/Valsartan/Adriamycin nephropathy/Apoptosis/Fas/FasL分类
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韩子明,邢燕,王宏伟,梁秀玲,周建华..缬沙坦对阿霉素肾病肾硬化大鼠细胞凋亡及Fas和FasL表达的影响[J].中国当代儿科杂志,2003,5(4):306-310,5.
