热带亚热带植物学报2002,Vol.10Issue(4):306-312,7.
红麻及其近缘种的RAPD分析
Random Amplified Polymorphic DNA (RAPD) Analyses among Hibiscus cannabinus and Related Species
郭安平 1周鹏 1粟建光2
作者信息
- 1. 中国热带农业科学院热带作物生物技术国家重点实验室,海南海口,571101
- 2. 中国农业科学院麻类研究所,湖南长沙,410006
- 折叠
摘要
Abstract
RAPDs were used to study the relationships among species in Hibiscus sect. Furcaria.Twenty-five accessions of 7 species were examined. Sixteen primers of arbitrary sequences were screened from 80 primers for DNA amplification. A total of 192 bands were generated, of which 149 bands were polymorphic markers. Dendrogram was constructed using Nei's genetic similarity value and MINTS program. The result showed that 25 accessions were clustered in 7 clades, H.penduriformis and H. calyphyllus formed a clade, and 15 accessions of H. cannabinus were clustered in two clades, one being the cultivars, and the other, the wild type materials. The rest 4 species formed 4 individual clades. The most parsimonious dendrogram shows that H. radiatus is closely related to H. sabdariffa. The closely related species to the cultivars of H. cannabinus are H. sabdariffa and H. radiatus, whereas H. trionum appears to be highly diverged from all the other taxa.关键词
红麻/近缘种/RAPD/亲缘关系Key words
Hibiscus cannabinus/Related species/RAPD/Phylogenetic relationship分类
农业科技引用本文复制引用
郭安平,周鹏,粟建光..红麻及其近缘种的RAPD分析[J].热带亚热带植物学报,2002,10(4):306-312,7.