中国临床康复2003，Vol.7Issue(22)：3071-3073,3.
基因bcl-2对神经元生物活性的保护作用
The protective effect to neuronal bioactivity by bcl-2 gene
吴永青 1刘彦文 2邱鹏新 3张清秀 4葛坚5
作者信息
- 1. 南京大学医学院附属鼓楼医院宁益眼科中心,江苏省南京市,210008
- 2. 中山医科大学动物实验中心,广东省广州市,510089
- 3. 中山医科大学药理学教研室,广东省广州市,510089
- 4. 中山医科大学生化教研室,广东省广州市,510089
- 5. 中山医科大学中山眼科中心,广东省广州市,510089
- 折叠
摘要
Abstract
AIM:To investigate the biological effects of bcl-2 gene on neurons and to provide effective methods for treatment for nervous system diseases. METHODS: The recombinant expression plasmid pcDNA3 bcl-2 was constructed from pSFFV bcl-2,then it was introduced into PC12 cell line by liposome method.Western blotting were applied to detect the exogenous gene expression.The two groups of cells(group A: PC12 transfected by pcDNA3 bcl-2 and group B:PC12 transfected by pcDNA3) were exposed to Cisplatin with the concentration of 10,50 and 100 μ mol/L.72 hours later, the survival cells in two groups were examined.Cell cycle indexes between these two groups were also studied by FCM. RESULTS:The recombinant expression plasmid pcDNA3 bcl-2 was constructed successfully and PC12 cell line transfected by the plasmid could express Bcl-2 protein effectively.After exposed to 10,50 and 100 μ mol/L Cisplatin,the surviving cells in group A were 276± 13,185± 11 and 108± 10 respectively, which increased much more than those in group B while they were 100± 9,12± 3 and 2± 2 accordingly and significant differences were found.Compared with the control(25.79% ), there was a significant decrease of cells from the S phase in PC12 with bcl-2 gene(8.81% ,χ 2 =22.53,P< 0.01). CONCLUSION:bcl-2 can protect PC12 cells against cytotoxic insults of cisplatin,the neuroprotective mechanism might be achieved via regulation on cell cycle. 关键词
基因,bcl-2/转染/PC12细胞/基因表达分类
医药卫生引用本文复制引用
吴永青,刘彦文,邱鹏新,张清秀,葛坚..基因bcl-2对神经元生物活性的保护作用[J].中国临床康复,2003,7(22):3071-3073,3.
