棉花学报2009，Vol.21Issue(4)：307-312,6.
大丽轮枝菌拮抗细菌B.subtilis LZ2-70产芽孢条件优化
Optimization of Spore Production Conditions of Antagonistic Bacteria Bacillus subtilis LZ2-70 Against Verticillium dahliae
摘要
Abstract
In order to improve the spore production rate and spore quantity of antagonistic strain Bacillus subtilis LZ2-70 against Verticillium dahliae , the main factors influencing spore production were investigated using shake-flask fermentation method.Through single factor experiment and orthogonal experiment, the optimal shaking flask fermentation condition of strain Bacillus subtilis LZ2-70 was determined as follows: media composed of 0.5% Maltose, 2% soybean meal, 0.05% KH2PO4 and 0.1% MnSO4·H2O, initial pH 8.0, culture time 24 h and bottle filling capacity 30 mL/250 mL, 10% inoculums volume, fermentation time 36 h, fermentation temperature 30℃, rotating speed 200 r·min-1.Under this optimum condition, the spore quantity of strain Bacillus subtilis LZ2-70 achieves 6.6×109·mL-1 and the spore production rate achieves 98.94%.关键词
大丽轮枝菌/拮抗细菌/枯草芽孢杆菌/发酵/芽孢Key words
Verticillium dahilae/antagonistic bacteria/Bacillus subtilis/fermentation/spore分类
农业科技引用本文复制引用
李术娜,王树香,王占利,李红亚,王全,朱宝成..大丽轮枝菌拮抗细菌B.subtilis LZ2-70产芽孢条件优化[J].棉花学报,2009,21(4):307-312,6.基金项目
河北省自然科学基金项目 （）
