首页|期刊导航|中华医学杂志（英文版）|Scanning electron microscopic observation: three-dimensional architecture of the collagen in hepatic fibrosis rats
中华医学杂志（英文版）2007，Vol.120Issue(4)：308-312,5.
Scanning electron microscopic observation: three-dimensional architecture of the collagen in hepatic fibrosis rats
Scanning electron microscopic observation: three-dimensional architecture of the collagen in hepatic fibrosis rats
摘要
Abstract
Background In the process of hepatic fibrosis, the accumulation of collagen fibers is strongly related to the hepatic function. The aim of this study was to investigate the three-dimensional architecture of the collagen network in the liver of rats with hepatic fibrosis.Methods Healthy adult male Wistar rats (n=32) were randomly divided into a control group (n=16) and a hepatic fibrosis group (n=16). In the control group, the rats were treated with peanut oil while the rats in hepatic fibrosis group were treated for 10 weeks with 60% CCl4 diluted in peanut oil. The quantity of collagen fibers was detected by Western blotting; distribution of the collagen was detected by sirius red staining and polarized microscope; the three-dimensional architecture of collagen in the liver was observed under the scanning electron microscope after fixed tissues were treated with cell-maceration using NaOH. Statistical analysis was performed using the u test.Results The quantity of collagen fibers increased significantly in the hepatic fibrosis group. With the aggravation of hepatic fibrosis, collagen fibers gradually accumulated. They interlaced the reticulation compartment and formed a round or ellipse liver tissue conglomeration like a grape framework that was disparate and wrapped up the normal liver Iobule.The deposition of collagen fibers was obvious in adjacent hepatic parenchyma, especially around the portal tracts.Conclusion Our experiment showed the collagen proliferation and displays clearly the three-dimensional architecture of collagen fibers in rat liver with hepatic fibrosis by scanning electron microscope. It can provide a morphological foundation for the mechanisms of changed haemodynamics and portal hypertension in hepatic fibrosis.关键词
hepatic fibrosis/ collagen fibers/ scanning electron microscope/ three-dimensional constructKey words
hepatic fibrosis/ collagen fibers/ scanning electron microscope/ three-dimensional construct分类
医药卫生引用本文复制引用
WANG Xiao-hong,ZHAO Jing,ZHANG Wei-guang,ZHANG Li-ying,MA Rui-qiong,WANG Li-qin,ZHANG Shu-yong,TIAN Long..Scanning electron microscopic observation: three-dimensional architecture of the collagen in hepatic fibrosis rats[J].中华医学杂志（英文版）,2007,120(4):308-312,5.基金项目
This study was supported by a grant from the National Natural Science Foundation of China (No. 30471638). （No. 30471638）
