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The Effect of Acid Stress Treatment on Viability and Membrane Fatty Acid Composition of Oenococcus oeni SD-2a

ZHAO Wen-ying LI Hua WANG Hua LI Zhong-chao WANG Ai-lian

中国农业科学(英文版)2009,Vol.8Issue(3):311-316,6.
中国农业科学(英文版)2009,Vol.8Issue(3):311-316,6.

The Effect of Acid Stress Treatment on Viability and Membrane Fatty Acid Composition of Oenococcus oeni SD-2a

The Effect of Acid Stress Treatment on Viability and Membrane Fatty Acid Composition of Oenococcus oeni SD-2a

ZHAO Wen-ying 1LI Hua 2WANG Hua 1LI Zhong-chao 1WANG Ai-lian1

作者信息

  • 1. College of Enology, Northwest A&F University, Yangling 712100, P.R.China
  • 2. College of Chemical Engineering and Environment, North University of China, Taiyuan 030051, P.R.China
  • 折叠

摘要

Abstract

To obtain ready-to-use wine malolactic starter cultures with high viability, the effects of acid stress treatments on the growth, inoculation viability, freeze-drying viability, and membrane fatty acid composition of the native Oenococcus oeni SD-2a strain were studied. The results showed that pH 3.5 and 3.2 adaptive treatments did not strongly decrease cell biomass but increased distinctly inoculation viability and freeze-drying viability. Concerning the membrane fatty acid composition, it was observed that acid stress conditions increased significantly the relative concentration of lactobacillic acid (C19cycl 1) and the unsaturated:saturated fatty acid ratio in cell membrane lipids. We assumed that acid-induced cross protective responses could be used in preparing ready-to-use O. oeni SD-2a malolactic starter cultures, and the accumulation of lactobacillic acid in the membrane of O. oeni SD-2a cells appears as an acid stress response mechanism,which might be related with the enhanced viability.

关键词

Oenococcus oeni/acid stress response/membrane fatty acid composition

Key words

Oenococcus oeni/acid stress response/membrane fatty acid composition

分类

农业科技

引用本文复制引用

ZHAO Wen-ying,LI Hua,WANG Hua,LI Zhong-chao,WANG Ai-lian..The Effect of Acid Stress Treatment on Viability and Membrane Fatty Acid Composition of Oenococcus oeni SD-2a[J].中国农业科学(英文版),2009,8(3):311-316,6.

基金项目

This work was financed by the Key Technology R&D Program of Shaanxi Province, China (2005K02-G2). (2005K02-G2)

中国农业科学(英文版)

2095-3119

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