医学分子生物学杂志2009，Vol.6Issue(4)：312-316,5.DOI:10.3870/j.issn.1672-8009.2009.04.007
草木犀石油醚提取物对小鼠巨噬细胞促炎介质的影响
Effect of Petroleum Ether Extract from Melilotus on Pro-inflam matory Mediators in Murine Macrophages
摘要
Abstract
Objective To study anti-inflammatory mechanism of petroleum ether extract from Melilotus suaveolens Ledeb in vitro.Method Inflammatory cell model was established by LPS acting on the RAW264.7 cell line. The secretion of TNF-α, IL-1β and IL-6 in supernatant was detected by ELISA. The mRNA expression of TNF-α, iNOS, COX-2 and the protein expression of COX-2 were detected by real-time PCR and Western blot respectively.Results The secretion levels of TNF-α, IL-1β, IL-6 and NO were significantly lower in the supernatant of MSL treated macrophages than those in controls (P<0.01). We also found significant differences among the mRNA expression of TNF-α, iNOS and COX-2 between the supernatant of MSL treated and untreated macrophages, as well as the protein expression of COX-2 (P<0.01).Conclusion The petroleum ether extract from Melilotus suaveolens Ledeb can significantly resist inflammation by inhibiting expression and secretion of pro-inflammatory mediators in murine macrophages treated by LPS.关键词
草木犀/石油醚/RAW 264.7细胞系/炎性介质Key words
Melilotus/petroleum ether/RAW264.7 cell line/mediators of inflammation分类
医药卫生引用本文复制引用
庞然,张淑玲,赵雷,易建华,董继华,刘双林..草木犀石油醚提取物对小鼠巨噬细胞促炎介质的影响[J].医学分子生物学杂志,2009,6(4):312-316,5.基金项目
湖北省自然科学基金(No:2007ABA080)This work was supported by a grant from the Natural Science Foundation of Hubei Province(No:2007ABA080) （No:2007ABA080）
