首页|期刊导航|中华医学杂志（英文版）|Role of nitric oxide and inducible nitric oxide synthase in human abdominal aortic aneurysms: a preliminary study
中华医学杂志（英文版）2006，Vol.119Issue(4)：312-318,7.
Role of nitric oxide and inducible nitric oxide synthase in human abdominal aortic aneurysms: a preliminary study
Role of nitric oxide and inducible nitric oxide synthase in human abdominal aortic aneurysms: a preliminary study
摘要
Abstract
Background Nitric oxide (NO) is an important mediator in the pathophysiology of many vascular diseases. However, the definite role of NO in human abdominal aortic aneurysm (AAA) formation is unclear. The aim of this study was to investigate production of NO and expression of inducible nitric oxide synthase (iNOS), and their possible role in AAA.Methods A total of 28 patients with AAA, 10 healthy controls, and 8 patients with arterial occlusive disease were enrolled into this study. Standard colorimetric assay was used to examine NO concentration in plasma from patients with AAA and normal controls, and in cultured smooth muscle cells (SMCs). Expression of iNOS in aortas and cultured SMCs were detected by immunochemistry. The correlation of iNOS expression with age of the patient, size of aneurysm, and degree of inflammation was also investigated by Cochran-Mantel-Haenszelχ2 test and Kendall' Tau correlation.Results Expression of iNOS increased significantly in the wall of aneurism in the patients with AAA compared to the healthy controls (P<0.05) and the patients with occlusive arteries (P<0.05). iNOS protein and media NOx (nitrite+nitrate) also increased in cultured SMCs from human AAA (n=4, P<0.05), while plasma NOx decreased in patients with AAA (n=25) compared to the healthy controls (n=20). There was a positive correlation between iNOS protein and degree of inflammation in aneurismal wall (Kendall coefficient=0.5032, P=0.0029)Conclusions SMCs and inflammatory cells were main cellular sources of increased iNOS in AAA, and NO may play a part in pathogenesis in AAA through inflammation.关键词
aortic aneurysm, abdominal/nitric oxide/nitric oxide synthase type IIKey words
aortic aneurysm, abdominal/nitric oxide/nitric oxide synthase type II分类
医药卫生引用本文复制引用
LIAO Ming-fang,LI Xiao-yan ,JING Zai-ping,BAO Jun-min,ZHAO Zhi-qing,MEI Zhi-jun,LU Qing-shen,Feng Xiang,FENG Rui,ZHANG Su-zen..Role of nitric oxide and inducible nitric oxide synthase in human abdominal aortic aneurysms: a preliminary study[J].中华医学杂志（英文版）,2006,119(4):312-318,7.基金项目
This study was supported by a grant from Clinical Medical Center of Vascular Surgery in Shanghai (NO. ZX03B1). （NO. ZX03B1）
