青岛大学医学院学报2001，Vol.37Issue(1)：31-32,2.
蜂毒肽表达克隆的初步构建
PRELIMINARY CONSTRUCTION OF MELLITIN EXPRESSION CLONE
摘要
Abstract
Objective To construct mellitin gene expression clone. Methods Two fragments of manmade oligonucleotide，A and B, were made into target gene by Klenow enzyme . Then ,the target gene and prokaryotic expression vector Pmal-p2 were restricted simultaneously and ligated by T4 DNA ligase. The recombinant plasmid was transformed into E.coli. DH5α. depending on the signs of α-complementary. We selected the positive clone and it was confirmed by restriction enzyme and DNA sequencing. Results The positive clone was identified with recombinant mellitin gene clone. Conclusion The construction of recombinant mellitin gene expression clone is the basis of mellitin protein expression and activity identification .关键词
蜂毒肽/基因/载体蛋白质类分类
生物科学引用本文复制引用
王秋波,吴虹,鲁迎年,张艳丽..蜂毒肽表达克隆的初步构建[J].青岛大学医学院学报,2001,37(1):31-32,2.
