中国人兽共患病杂志2000，Vol.16Issue(6)：31-32,27,3.
弓形虫P22编码基因真核表达质粒接种小鼠后的细胞免疫效果
STUDY ON CELLULAR IMMUNE RESPONSE INDUCED BY IMMUNIZATION WITH A DNA PLASMID ENCODING P22 PROTEIN OF TOXOPLASMA GONDII IN MICE
高世同 1吴少庭 1林敏 1占国清 2郑春福3
作者信息
- 1. 深圳市卫生防疫站深圳，518020
- 2. 同济医科大学硕士生
- 3. 重庆医科大学博士生
- 折叠
摘要
Abstract
In order to study the cellular response induced by immunization with the DNA plasmid (pBK/p22)encoding the P22 protein of Toxoplasma gondii in mice. After 5 weeks of vaccination,the proliferation activity of spleen T lymphcytes was dectect by MTT assay and the number of CD+4/CD+8 by indirect immunofluorescent assay. The results of MTT assays had no significant difference among pBK/P22 group,pBK-CMV empty plasmid group and NS control group(P＞0. 05). The number of CD+4T cells shows no obvious increase compared with pBK-CMV and NS groups(P＞0. 05). However,CD+8T cell was significant increased (P＜0.01) and the value of CD+4/CD+8 was decreased.关键词
弓形虫/P22编码基因/DNA接种/细胞免疫Key words
Toxoplasma gondii P22gene DNA vaccination Cellular immune分类
医药卫生引用本文复制引用
高世同,吴少庭,林敏,占国清,郑春福..弓形虫P22编码基因真核表达质粒接种小鼠后的细胞免疫效果[J].中国人兽共患病杂志,2000,16(6):31-32,27,3.
