中国医科大学学报2001，Vol.30Issue(1)：31-32,40,43,4.
提纯G3PD的修饰蛋白
Purify a Modifier Protein of Glyceraldehyde-3-Phosphate Dehydrogenase
任青 1严海东 1吴岚 1李子龙 1马健飞 1王力宁 1周希静1
作者信息
摘要
Abstract
Objective： Our aim was to purity the modifier protein of glyceraldehyde-3-phosphate dehydrogenase (G3PD) from African green monkey Vero-E6 line. Methods：Exposure of Vero-E6 cells to medium with a reduced K concentration (3.2 mmol/L) stimulated the growth and activation of G3PD. The increase of enzyme activity was mediated by a cytosolic modifier protein that was purified using affinity and anion-exchange high-performance liquid chromatograph. Results：The apparent molecular mass of the protein was 62 kDa. Western blotting and quantiative enzyme-linked immunosorbent assay showed that the amount of modifier protein increased progressively for 2 hours in cells exposed to low-K+ medium, and then returned to the control value, a kinetic profile similar to that the modifier protein is a constituent of renal epithelial cells and accummulated transiently in the low-K+ mitogenic signal. Conclusion: We obtained a modifer protein from monkey kidney epithelial cells (Vero-E6). It could activate G3PD and cell growth.关键词
Vero-E6细胞/钾缺乏/修饰蛋白/3-磷酸甘油醛脱氢酶/细胞增殖分类
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任青,严海东,吴岚,李子龙,马健飞,王力宁,周希静..提纯G3PD的修饰蛋白[J].中国医科大学学报,2001,30(1):31-32,40,43,4.
