中国动脉硬化杂志2001，Vol.9Issue(1)：31-33,3.
氧化型低密度脂蛋白对培养的内皮细胞的致凋亡作用及尼莫地平的保护作用
Proatherogenic Effect of Oxidized Low Density Lipoprotein on Cultured Endothelium Cells and Protect Effect of Nimodipine
郭素箴 1刘克强 1齐新 1黄繁嫱2
作者信息
- 1. 天津市第一中心医院心内科
- 2. 检验科， 天津市 300192
- 折叠
摘要
Abstract
Aim To elucidate the proatherogenic effect of oxidized lowdensity lipoprotein (ox-LDL)on the apoptosis of cultured endothelium cells and protect effect of nimodipine. Methods ox-LDL was obtained by incubated with Cu2+,different concentrations of ox-LDL incubated with endothelium cells. MTT test was carried out to evaluate the cell growing state. PI staining test and ELISA test were conducted to analyze apoptosis index. Results The inhibiting effect of ox-LDL on the endothelium growth by ox-LDL was in a dose- dependent manner. ELISA and PI staining showed that ox-LDL induced endothelium cell apoptosis at the same concentration (P<0.05), Nimodipine could inhibited the ox-LDL induced endothelium cell apoptosis. Conclusions The cytotoxicity of ox-LDL to endothelium cell was related to the dosage concentration of ox-LDL: the ox-LDL could inhibit the cultured endothelial cells growth,and even resulted in apoptosis. ox-LDL could start the atherosclerosis in vivo due to the endothelium cells impairment and apoptosis.Nimodipine could protect endothelium from ox-LDL induced apoptosis.关键词
脂蛋白,低密度,氧化型/内皮,血管/尼莫地平/凋亡分类
医药卫生引用本文复制引用
郭素箴,刘克强,齐新,黄繁嫱..氧化型低密度脂蛋白对培养的内皮细胞的致凋亡作用及尼莫地平的保护作用[J].中国动脉硬化杂志,2001,9(1):31-33,3.
