兰州大学学报（医学版）2009，Vol.35Issue(2)：31-35,5.
兰州羔羊轮状病毒株VP4基因稳定性及基因结构分析
Analysis of VP4 gene stability and structure of Lanzhou lamb rotavirus strain
刘晨鸣 1高雪军 1赵雅静 1魏至栋 1冯德杰 1朱莉萍1
作者信息
- 1. 兰州生物制品研究所,质量检定室,甘肃,兰州,730046
- 折叠
摘要
Abstract
Objective To study the characteristics of genetic variation of VP4 gene of Lanzhou lamb rotavirus (LLR) strain of rotavirus and its association with the genetic structure of human rotavirus in order to provide theoretic evidence for the vaccine quality control and development. Methods LLR38 strain was amplified in primary fetal calf kidney cells for 38～49 generations according to the procedure of vaccine production,and LLR38,LLR43,LLR44 and LLR49 were analyzed. The cDNA of VP4 gene was obtained by reverse transcription polymerase chain reaction (RT-PCR),the recombinant plasmid was constructed and the cloned VP4 gene was sequenced. Results The whole length of VP4 gene of LLR strain was 2 362 bp encoded 776 amino acids. Sequence analysis indicated that there was high genetic stability among the sequences of every passage. The homology of nucleotide or amino acid was 99.7%～100.0% or 99.0%～100.0%,respec- tively. The analyzing result also indicated that there were no evident changes in their phylogenetic relatives between different kinds of P types and the original rotavirus. Conclusion VP4 gene of the LLR strain has good genetic stability,its vaccine is safe and may be used for the prevention of retrovirus infection.关键词
轮状病毒/兰州羔羊轮状病毒株/VP4基因/逆转录-聚合酶链反应/序列分析Key words
rotavirus/Lanzhou lamb rotavirus strain/VP4 gene/reverse transcription poly-merase chain reaction/sequence analysis分类
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刘晨鸣,高雪军,赵雅静,魏至栋,冯德杰,朱莉萍..兰州羔羊轮状病毒株VP4基因稳定性及基因结构分析[J].兰州大学学报（医学版）,2009,35(2):31-35,5.
