生命科学研究2003，Vol.7Issue(4)：315-319,5.
重组人α降钙素基因相关肽在大肠杆菌中表达条件的优化
Optimization of Recombinant Human α Calcitonin Gene-related Peptide Expression in E. Coli
摘要
Abstract
In order to improve the expression level of human α calcitonin gene-related peptide (hαCGRP), the growth conditions of the engineering strain, that confirmed by transforming the recombinant plasmid pET-hαCGRP which constructed by our team into the host E. coli BL21trxB(DE3)pLysS were studied, which remarkably influenced the final yield of protein expression. With the aid of SDS-PAGE and YLN2000 gel image analysis, it was found that the best expressed condition was that the induction was started as OD600 reached 0.6 ～ 0.8 by adding IPTG to a final concentration of 0.75 mmol/L and then continued incubation 4 hours at 37 ℃ (180 + 5 r/min) in the LB medium containing 50 mg/L ampicillin with shaking flasks. The maximum yield of fusion protein was about 70％ ～ 80％ of the total mass of bacterial proteins. Furthermore the soluble form of the target protein makes convenience for next purification work.关键词
人α降钙素基因相关肽/表达条件/优化/SDS-聚丙烯酰胺凝胶电泳Key words
hαCGRP/expression condition/optimization/SDS-PAGE分类
生物科学引用本文复制引用
赵丹丹,于晓光,班莺,邹海峰,林平,徐华锋..重组人α降钙素基因相关肽在大肠杆菌中表达条件的优化[J].生命科学研究,2003,7(4):315-319,5.基金项目
黑龙江省自然科学基金项目(D01-55)和哈尔滨市学科后备带头人基金项目(0171218001) （D01-55）
