首页|期刊导航|中国农业科学（英文版）|Generation of Recombinant Equine Influenza Vaccine Candidate RgH3N1 Virus by Reverse Genetics
中国农业科学（英文版）2005，Vol.4Issue(4)：317-320,4.
Generation of Recombinant Equine Influenza Vaccine Candidate RgH3N1 Virus by Reverse Genetics
Generation of Recombinant Equine Influenza Vaccine Candidate RgH3N1 Virus by Reverse Genetics
摘要
Abstract
The antigenic variation of influenza A virus hemagglutinin (HA) glycoproteins requires frequent changes in vaccine formulation. The new strategy of creating influenza seed strains for vaccine production is to generate 7 + 1 reassortants that contain seven genes from a high-yield virus A/Puerto Rico/8/34[A/PR/8/34](H1N1) and the HA gene from the circulating strains. By using this DNA-based cotransfection technique, we generated 7 + 1 reassortants rgH3N1 which had the antigenic determinants of influenza virus A/Songbird/HongKong/102/00[SB/HK/01](H3N8) and 7 other genes from A/PR/8/34. The hemagglutinin of A/Songbird/HongKong/102/00 is 96.3% homologous to that of A/Equine/Jilin/98[Eq/J1/89] (H3N8). The resulting virus rgH3N1 grows to high HA titers in chicken embryonated eggs, allowing vaccine preparation in unconcentrated allantoic fluid. The rgH3N1 is stable after multiple passages in embryonated eggs. The reassortant rgH3N1 virus could be used as vaccine candidate to reduce the reemergence of equine influenza outbreaks.关键词
Influenza A virus/Reverse genetics/In vitro properties/Stability of reassortant virusKey words
Influenza A virus/Reverse genetics/In vitro properties/Stability of reassortant virus引用本文复制引用
ZHANG Yun,LIU Ming,YU Kang-zhen,Webster Robert..Generation of Recombinant Equine Influenza Vaccine Candidate RgH3N1 Virus by Reverse Genetics[J].中国农业科学（英文版）,2005,4(4):317-320,4.基金项目
This work was supported by grant from National Institute of Allergy and Infectious Diseases Public Health Service Research (AI95357). （AI95357）
