中国临床康复2003，Vol.7Issue(23)：3174-3175,2.
人胚胎嗅鞘细胞植入鼠半横断脊髓的初步观察
Preliminary observation of the effect of transplantation of human embryonic OECs on adult SD rats' semitransected spinal cord
摘要
Abstract
AIM:To observe the effect of transplantation of human embryonic OECs on adult rats' semitransected spinal cord. METHODS: OECs,separated from human embryonic olfactory bulb and purified by culture in vivo for 2 weeks,were transplanted into SD rats'(n=10) semitransected spinal cord at T10 level,while the vehicle solution (DMEM) was injected in the controls(n=10).Samples were procured at 6 week after transplantation and studied by nerve silver staining and myelin basic protein(MBP) histochemical staining and nerve growth factor receptor(NGFR) immunohistochemical staining. RESULTS:The injured spinal cords displayed initial healing in OECs transplanted rats 6 weeks post-transplantation morphologically.Histological observation showed that the transplanted OECs integrated into the host tissues in well arrangement and the regenerative axons grew into the distal end of the semitransected spinal cords.Both of OECs and regenerative axons positively expressed MBP and NGFR.In control group, the ends of the impaired spinal cords exhibited liquefied necrosis and axonal degeneration and no regnnerative axons were found. CONCLUSION:OECs transplantation could protect the impaired spinal cord and promote the regeneration of axons.关键词
嗅球/神经鞘/细胞/脊髓损伤/轴突/再生分类
医药卫生引用本文复制引用
王学文,唐勇,吴燕峰,殷德振,杨睿,黄霖,沈慧勇..人胚胎嗅鞘细胞植入鼠半横断脊髓的初步观察[J].中国临床康复,2003,7(23):3174-3175,2.基金项目
国家自然科学基金 (30271311) （30271311）
广东省科技厅攻关项目 (C30702) （C30702）
