中国临床康复2004，Vol.8Issue(16)：3176-3177,2.
肌苷对大鼠脑缺血再灌注后血管内皮生长因子表达的影响
Effect of inosine on expression of vascular endothelial growth factor following local cerebral ischemic reperfusion in rats
摘要
Abstract
BACKGROUND: Inosine takes part in a lot of metabolic processes and has a protective effect on cerebral ischemic injury, but its mechanism is not known thoroughly.OBJECTIVE: To study the effect of inosine on expression of vascular endothelial growth factor(VEGF) in the cerebral tissue after cerebral ischemia reperfusion in rats, and to explore the neuroprotective mechanism of inosine.DESIGN: A randomized controlled basic research.SETTING and MATERIALS: This experiment was carried out at the Institute of Cerebrovascular Diseases, College of Medicine, Qingdao University and the Key Laboratory of Cerebrovascular Diseases of Shandong Province.Sixty-eight adult healthy female SD rats, weighting 230- 270 g, clearing grade, were purchased from Shanghai Experimental Animal Center of the Chinese Academy of Science.INTERVENTION: The models of ischemia/reperfusion in SD rats were established by middle cerebral artery occlusion(MCAO) with a nylon monofilament suture. The rats were randomly divided into the treatment groups 32cases and the control group 32 cases. Each group was divided into eight subgroups, which consisted of 4 rats at 2 hours, 6 hours, 12 hours, 24 hours, 2days , 3 days, 7 days and 14 days after reperfusion of MACO. The other 4cases served as sham-operated group. Immunohistochemical technique was used to investigate the dynamic changes of VEGF in cerebral tissue.RESULTS: There was no expression of VEGF in cerebral tissue of sham-operated group. The expression of VEGF occurred at 2 hours, peaked at 12-24 hours after reperfusion and then decreased rapidly in cortex and striatum in the control group. VEGF expressed mainly in neurons and endothelial cells in Ⅱ, Ⅲ, Ⅳ layers in cortex, especially colored mostly in the perinucleus cytoplasm and dendrites of neurons. In the treatment group, the level of VEGF expression was significantly higher than that in the control group at reperfusion 2 hours - 2 days( t = 3.78 - 22.62, P ＜ 0.01 ).CONCLUSIONS: These results indicated that Inosine could protect cerebral tissue against hypoxic-ischemic injury after reperfusion of focal cerebral ischemia, whose role might be reflected by increasing the expression of VEGF.关键词
脑缺血/病理生理学/肌苷/治疗应用/内皮生长因子/基因表达分类
医药卫生引用本文复制引用
张红,李琴,谭金强,宫钦爽,郭云良..肌苷对大鼠脑缺血再灌注后血管内皮生长因子表达的影响[J].中国临床康复,2004,8(16):3176-3177,2.基金项目
山东省自然科学基金资助项目(Y2001C04) （Y2001C04）
