首页|期刊导航|高技术通讯（英文版）|A gene encoding AtPIP5K2 may be involved in regulating the sensitivity to osmotic stress
高技术通讯（英文版）2006，Vol.12Issue(3)：318-322,5.
A gene encoding AtPIP5K2 may be involved in regulating the sensitivity to osmotic stress
A gene encoding AtPIP5K2 may be involved in regulating the sensitivity to osmotic stress
摘要
Abstract
One mutant line eto with salt tolerance was screened from a T-DNA insertion mutant collection of Arabidopsis thaliana. In addition to a reduced rate of seed germination, NaCl and ABA also inhibited the growth and the greening of cotyledons of wild-type seedlings, but not the eto mutant. TAIL-PCR analysis showed that T-DNA tag insertion in the eto was located at nucleotide 27,502 in BAC F3M18, upstream (at position -487 relative to the translation initiation codon) of gene At1g77740 (encoding a putative phosphatidylinositol-4-phosphate 5-kinase, AtPIP5K2). This inserted mutation cosegregated closely with the eto phenotype. Another analysis not only indicated that AtPIP5 K2 transcript is expressed predominantly in roots and rosette leaves, but also showed the T-DNA insertion resulted higher accumulation of the AtPIP5K2 in eto mutant plants and did not influenced the expression of the upstream At1g77730 gene. This change may play an essential role in the tolerance of eto mutant plant to the osmotic stress.关键词
Arabidopsis thaliana, T-DNA, mutant, osmotic stress, phosphatidylinositol-4-phosphate 5-kinaseKey words
Arabidopsis thaliana, T-DNA, mutant, osmotic stress, phosphatidylinositol-4-phosphate 5-kinase分类
信息技术与安全科学引用本文复制引用
Song Yingqi,Yang Qian,Qin Genji,Qu Lijia..A gene encoding AtPIP5K2 may be involved in regulating the sensitivity to osmotic stress[J].高技术通讯（英文版）,2006,12(3):318-322,5.基金项目
Supported by the National Program for Transgenic Plants from China ( GN. J99-A-001 ) and National Natural Science Foundation of China ( GN.30221120261 ). （ GN. J99-A-001 ）
