中国临床康复2004，Vol.8Issue(16)：3207-3209,3.
二氧化硫衍生物对大鼠海马神经元外向钾电流特性的影响
Influence of Sulfur dioxide derivatives on outward potassium current in rat hippocampal neurons
桑楠 1孟紫强1
作者信息
- 1. 山西大学环境与资源学院,环境医学与毒理学研究所,山西省太原市,030006
- 折叠
摘要
Abstract
BACKGROUND: Sulfur dioxide (SO2) and its derivatives are recognized as the factors responsible for cellular chromosome cleavage and genotoxicity and capable of inducing oxidative injury and changes in enzyme activity. The toxicity of SO2 and its' derivatives, from the perspective of cell membrane injuries and neurotoxic effects in particular, remains currently unclear.OBJECTIVE: To study the characteristics of depolarization-activated potassium current in rat hippocampal neurons, and observe the changes in the current in response to cell treatment with SO2 and its derivatives.DESIGN: Completely randomized self-controlled study.SETTING and PARTICIPANTS: This study was conducted in the Institute of Environmental Medicine of Shanxi University. Forty 7-day-old Wistar rats weighing 8 to 10 g of either sex were provided by the Experimental Animal Center of China Institute for Radiation Protection.INTERVENTION: Whole-cell patch-clamp technique was used.MAIN OUTCOME MEASURE: Outward current elicited by transient depolarization to over - 60 mV was recorded and the changes in the amplitude of the current were observed after stimulation with S02 derivatives.RESULTS: Transient depolarization to over - 60 mV could induce rapidly increased outward current, which slowly attenuated to a plateau level. Changes in the holding potentials elicited peak and plateau current changes, but their amplitude changes varied, indicating that the outward current consisted two components. The reversal potentials of the peak and plateau currents were - 76. 1 +5.97 and -83.6 + 4, 13 mV, respectively, similar to potassium equilibrium potential(Ek = -88 mV) between extracelluar and intra-electrode fluid calculated by Nerst equation, suggesting that the outward current was potassium current. SO2 derivatives could amplify the two components of the outward K +current dose-dependently, and their doses for 50% increment of the two components were 26. 19 and 14.50μmol/L respectively.CONCLUSION: Sulfur dioxide derivatives can anplify voltage-dependent outward K + current in rat hippocampal neurons to cause central nervous system injury as a result of excessive intracellular potassium outflow through K+channels and intraeellular K + loss.关键词
海马神经元/钾/二氧化硫/细胞膜分类
医药卫生引用本文复制引用
桑楠,孟紫强..二氧化硫衍生物对大鼠海马神经元外向钾电流特性的影响[J].中国临床康复,2004,8(16):3207-3209,3.
