中国现代医学杂志2006，Vol.16Issue(3)：321-324,328,5.
环氧合酶-2在星形细胞肿瘤组织中的表达及临床意义
Expression of cyclooxygenase-2 in astrocytic tumours and its clinical significance
冯天达 1刘岿然 2周欣 2王成林 1于宏伟1
作者信息
- 1. 中国医科大学第二临床学院神经外科,辽宁,沈阳,110004
- 2. 中国医科大学第二临床学院妇产科,辽宁,沈阳,110004
- 折叠
摘要
Abstract
[Objective] To investigate the relationship between cyclooxygenase-2(COX-2) and occurrence and development of astrocytic tumours. [Methods] Immunohistochemistry technique and reverse transcription polymerase chain reaction (RT-PCR) technique were adopted to examine the levels of expression of COX-2 protein and mRNA in astrocytic tumours and non-tumour tissues. [Results] The expression of COX-2 protein in each group by immunohistochemical staining (IODA) was: non-tumour tissues(2.19±0.88), astrocytic tumours(6.38±3.68), pilocytic astrocytoma (3.54±1.29), diffuse astrocytoma (5.58±2.78), anaplastic astrocytoma (6.88±2.70), glioblastoma (9.19±4.62); the rate of positive expression of COX-2 mRNA was: non-tumour tissues (33.3%), astrocytic tumours (83.3%); the relative content of COX-2 mRNA was: non-tumour tissues (43.49± 19.73), astrocytic tumours ( 119.67±46.56); the expression of COX-2 in astrocytic tumours was significantly highter than in non-tumour tissues (P ＜0.05). [Conclusions] The over-expression of COX-2 has close relation with occurrence and development of astrocytic tumours; Pilocytic astrocytoma is different from other astrocytic tumours in change of gene in biology and morphology; COX-2 has complicated action in the course of organ growth and maintaining normal physiological function besides COX-2 joins the pathological courses of tumourigenesis and inflammatory occurrence etc.关键词
星形细胞肿瘤/环氧合酶-2/免疫组化/逆转录聚合酶链反应Key words
astrocytic tumou rs/COX-2/immunohistochemistry/RT-PCR分类
医药卫生引用本文复制引用
冯天达,刘岿然,周欣,王成林,于宏伟..环氧合酶-2在星形细胞肿瘤组织中的表达及临床意义[J].中国现代医学杂志,2006,16(3):321-324,328,5.
