首页|期刊导航|哈尔滨工业大学学报（英文版）|Hydrogen producting characteristics by a novel strain of bacteria-ethanoligenens sp. B49
哈尔滨工业大学学报（英文版）2008，Vol.15Issue(3)：321-325,5.
Hydrogen producting characteristics by a novel strain of bacteria-ethanoligenens sp. B49
Hydrogen producting characteristics by a novel strain of bacteria-ethanoligenens sp. B49
摘要
Abstract
The objective of this work is to investigate the fermentation capacity and metabolic characteristics of a novel strain of bacteria B49 isolated from anaerobic activated sludge. The examination was conducted in batch culture at 35 ℃. The results showed that the carbon flow gave priority to the production of ethanol, and yield of ethanol is always greater than that of acetic acid. The hydrogen and ethanol occurred simultaneously. The exponential phase of the B49's cell growth was from 12 to 22 h. Evolution of hydrogen appeared to start after the exponential phase of cell growth and reach maximum production at the early stationary phase. The rate of hydrogen production reached a maximum of 16.8 mL/h, and the percentage of hydrogen gas in the headspace of serum bottle obtained a maximum of 41% at 22 h. The B49 was able to grow using molasses as substrate for cell growth. When the molasses was used as substrate, maximum yield of hydrogen was obtained 2460 mL/L culture at 2% (V/V) of molasses. The hydrogen yield was increased to 3060 mL/L culture after addition of 0.5 g/L of yeast extract in the molasses medium and the yield of hydrogen was increased by 24.4%.关键词
biohydrogen production/ ethanol/ dynamics/ molasessKey words
biohydrogen production/ ethanol/ dynamics/ molasess分类
资源环境引用本文复制引用
XU Li-ying,REN Nan-qi,WANG Xing-zu,ZHANG Ying,XU Hui,CHEN Guan-xiong,JIA Yong-feng..Hydrogen producting characteristics by a novel strain of bacteria-ethanoligenens sp. B49[J].哈尔滨工业大学学报（英文版）,2008,15(3):321-325,5.基金项目
Sponsored by the National Natural Science Fundation of China(Grant No.30470054). （Grant No.30470054）
