中德临床肿瘤学杂志（英文版）2007，Vol.6Issue(4)：322-324,3.
TSLC1基因真核表达载体的构建及在HepG2细胞中的表达
Construction of eukaryotic expression vector carrying human TSLC1 gene and its expression in HepG2 cells
Li Qin 1Yongjun Tian 1Zhengmao Zhang 1Youhua Hao 1Dongliang Yang1
作者信息
- 1. Division of Clinical Immunology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China
- 折叠
摘要
Abstract
Objective: To construct the eukaryotic expression vector for human TSLC1 gene, and to express TSLC1 in HepG2 cells for investigating its effect on HepG2 cell growth. Methods: Full length of TSLC1 cDNA was amplified from RNA of normal human liver by RT-PCR, and cloned into pCI-neo expression vector. The recombinant plasmid pCI-TSLC1 was identified with restriction enzyme and sequenced, and then was stably transfected into HepG2 cells with lipofectamine 2000.The positive clones were examined by western-blotting and immunofluorescence, cell growth was analyzed with MTT assay.Results: The eukaryotic expression vector pCl-TSLC1 was successfully constructed and the stable cell line highly expressing TSLC1 protein was obtained. The growth of TSLC1-transfected cells was significantly suppressed in vitro. Conclusion: The HepG2 stable cell line could highly express TSLC1 protein, which provided a basis for further exploring the roles of TSLC1 in hepatocellular carcinoma.关键词
TSLC1 gene/eukaryotic expression/carcinoma, hepatocellularKey words
TSLC1 gene/eukaryotic expression/carcinoma, hepatocellular分类
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Li Qin,Yongjun Tian,Zhengmao Zhang,Youhua Hao,Dongliang Yang..TSLC1基因真核表达载体的构建及在HepG2细胞中的表达[J].中德临床肿瘤学杂志（英文版）,2007,6(4):322-324,3.
