第一军医大学学报2001，Vol.21Issue(1)：32-33,36,3.
混合培养神经元和星形胶质细胞对活性氧的应激性
Excitability of cocultured cerebral cortex neurons and astrocytes to tert-butyl hydroperoxide cytotoxicity
莫永炎 1陈瑗 1周玫 1张宝 1邱小忠1
作者信息
摘要
Abstract
Objective To investigate the response of neurons and astrocytesfrom Sprague Dawley rat brain against tert-buty1 hydroperoxide(tbOOH) toxicity,both separately and in cooperation, for the purpose of exploring the interrelation of the two kinds of cells. Methods Cell viability of singly cultured neurons or astrocytes from the cerebral cortex was compared by MTT reduction assay,and the morphological changes of cocultured neurons and astrocytes were observed upon tbOOH attack. Results The difference of cell viability was insignificant(P>0.05) between the neurons and astrocytes, but the damage of the neurons exceeded that of the astrocyte in terms of severity in mixed culture. Conclusion The astrocytes is very likely to possess better ability to fight tbOOH toxicity than the neurons do when they are cultured together, suggesting that the protection of neuron against active oxygen species toxicity is actually executed by the astrocyte.关键词
大脑皮质/神经元/星形神经胶质细胞/叔丁基脂氢过氧化物/氧应激分类
医药卫生引用本文复制引用
莫永炎,陈瑗,周玫,张宝,邱小忠..混合培养神经元和星形胶质细胞对活性氧的应激性[J].第一军医大学学报,2001,21(1):32-33,36,3.
